|• Over 10 years' experience for 6000+ recombinant proteins production.
• One-stop service from gene to protein.
• Multiple protein expression systems: bacterial, yeast, baculovirus-insect and mammalian expression system.
• Multiple purification systems (30+) to choose.
• Abundant R & D ability with over 1000 new proteins per year and quick troubleshooting.
• Close to 100 bioreactors with various volumes ranging from 2 to 1000 L to meet high-throughput and large-scale production.
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|E.coli protein expression service||• Over 10 years' experience of protein expression|
|• Multiple protein purification and refolding technologies|
|• High-throughput and large-scale protein expression and production|
|• One-stop service from gene to purified protein|
|• Multiple expression vectors and E.coli cells to meet various expression needs|
|Mammalian transient protein expression service||• High-efficiency expression vectors|
|• Optimized mammalian expression systems|
|• High-throughput and large-scale production|
|• High yield expression with post-translational modification and correct folding|
|• One-stop service from gene sequence to purified protein|
|Baculovirus-Insect Cell Protein Expression Service||• High-efficiency expression of intracellular and extracellular proteins|
|• Multiple insect cells: Sf9，Sf21，Hi-5|
|• High-throughput and large-scale protein production|
|• Optimized expression vectors and higher-titre virus packaging|
|Advantages||• low cost
• rapid expression
• easy scale-up
• most widely used system for recombinant protein production
|• low cost
• rapid expression
• high yield
• diverse post-translational modifications
|• high capacity genes
• soluble proteins
• suitable for toxic proteins
• post-translational modifications similar to mammalian systems
|• soluble protein
• lower endotoxin
• better bioactivity
• comprehensive post-translational modifications
• transient & stable expression
|Challenges||• inclusion bodies
• lack of eukaryotic post-translational modifications
• difficult to express higher MW proteins
|• improper glycosylation
• high mannose modification
|• more demanding culture conditions
• lack of partial glycosylation
|• more demanding culture conditions|
|Most common applications||• bacterial proteins
• antigen proteins
• lower MW proteins
|• cytoplasmic proteins
• toxic proteins
• transmembrane proteins
• secretory proteins
|• secretory proteins
• extracellular domain of transmembrane proteins
• recombinant antibodies
• antibody fragments