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Maus VAPA Gene ORF cDNA clone in cloning vector

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Maus VAPA Produktinformation zum cDNA-Klon
Gene_bank_ref_id:NM_013933.3
cDNA-Größe:750bp
cDNA-Beschreibung:Full length Clone DNA of Mus musculus vesicle-associated membrane protein, associated protein A.
Synonyme für Gene:33kDa, VAP33
Spezies:Mouse
Vektor:pGEM-T Vector
Plasmid:pGEM-mVAPA
Restriktionsschnittstelle:
Tag-Sequenz:
Sequenzbeschreibung:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
( We provide with VAPA qPCR primers for gene expression analysis, MP202496 )
Promoter:
Application:
Antibiotic in E.coli:Ampicillin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Lagerung:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
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Katalog: MG52625-G
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