Anti-UCHL3 Antibody

Cat: 80087-RP02

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All UCHL3 Reagents

Anti-UCHL3 Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-UCHL3 Antibody
Species reactivity
Reacts with: Rat
Recombinant Rat UCHL3 protein (Catalog#80087-R07E)
Produced in rabbits immunized with purified, recombinant Rat UCHL3 (rR UCHL3; Catalog#80087-R07E; Q91Y78; Glu 2-Ala 230). UCHL3 specific IgG was purified by Rat UCHL3 affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-UCHL3 Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-UCHL3 Antibody (Rabbit Polyclonal antibody) Images

Rat UCHL3 Immunoprecipitation(IP) 6103

rat UCHL3 was immunoprecipitated using:

Lane A:0.5 mg K562 Whole Cell Lysate

Lane B:0.5 mg Jurkat Whole Cell Lysate

0.5 µL anti-rat UCHL3 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-rat UCHL3 rabbit polyclonal antibody,at 1:1000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 26 kDa

Observed band size: 26 kDa

Rat UCHL3 Western blot (WB) 13738

Anti-UCHL3 rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:26 kDa

Observed band size:23 kDa

(We are unsure as to the identity of these extra bands.)

UCHL3 Background Information

Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCH-L3, Ubiquitin thioesterase L3 and UCHL3, is a ubiquitin-protein hydrolase which belongs to the peptidase C12 family. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCHL3 is highly expressed in heart, skeletal muscle, and testis. UCHL1 and UCHL3 are two of the deubiquitinating enzymes expressed in the brain. These phenotypes indicate the importance of UCHL1 and UCHL3 in the regulation of the central nervous system. UCHL3 functions as a de-ubiquitinating enzyme where lack of its hydrolase activity may result in the prominent accumulation of ubiquitinated proteins and subsequent induction of stress responses in skeletal muscle. UCHL3 has also been identified as a tumor-specific antigen in colon cancer.
Full Name
ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
  • Wood,M.A. et al., 2005, Hippocampus  15 (5):610-21.
  • Kwon,J. et al., 2006, Exp Anim  55 (1):35-43.
  • Setsuie,R. et al., 2009, Neurochem Int  54 (5-6):314-21.
  • Setsuie,R. et al., 2010, Neurochem Int  56 (8):911-8.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-UCHL3 Antibody Rabbit Monoclonal WB,ELISA,IHC-P,IP 80087-R007
    Anti-UCHL3 Antibody Rabbit Monoclonal ELISA 80087-R028
    Anti-UCHL3 Antibody Rabbit Polyclonal ELISA 50692-RP01
    Anti-UCHL3 Antibody Rabbit Monoclonal ELISA 50692-R033
    Anti-UCHL3 Antibody Rabbit Monoclonal WB,IP 50692-R153
    Anti-UCHL3 Antibody Rabbit Monoclonal IHC-P 50692-R204
    Anti-UCHL3 Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 50692-RP02
    Anti-UCHL3 Antibody Rabbit Monoclonal ELISA 11634-R001
    Anti-UCHL3 Antibody Rabbit Polyclonal WB,ELISA,IP 11634-RP02
    Anti-UCHL3 Antibody Rabbit Polyclonal ELISA 11634-RP01
    Anti-UCHL3 Antibody Rabbit Polyclonal WB,IHC-P 107313-T40
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