Anti-CD98 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-CD98 Antibody
Applications
WB,ELISA,IHC-P
Species reactivity
Reacts with: Mouse
Immunogen
Recombinant Mouse SLC3A2 protein (Catalog#50813-M07H)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse SLC3A2 (rM SLC3A2; Catalog#50813-M07H; NP_001154885.1; Ala 139-Ala 565). SLC3A2 specific IgG was purified by Mouse SLC3A2 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Anti-CD98 Antibody (Rabbit Polyclonal antibody) Validated Applications
| Application |
Dilution |
Notes |
| WB |
1:500-1:1000 |
|
| ELISA |
1:25000-1:50000 |
|
| IHC-P |
1:1000-1:5000 |
|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-CD98 Antibody (Rabbit Polyclonal antibody) Images
Immunochemical staining of mouse SLC3A2 in mouse kidney with rabbit polyclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to nephric tubule. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Anti-SLC3A2 rabbit polyclonal antibody at 1:500 dilution.
Lane A: K562 Membrane Lysate
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Predicted band size:68 kDa
Observed band size:68 kDa
(We are unsure as to the identity of these extra bands.)
Anti-CD98 Antibody Alternative Names
Anti-4F2 Antibody;Anti-4F2HC Antibody;Anti-AI314110 Antibody;Anti-Cd98 Antibody;Anti-Ly-10 Antibody;Anti-Ly-m10 Antibody;Anti-Ly10 Antibody;Anti-Mdu1 Antibody;Anti-Mgp-2hc Antibody;Anti-NACAE Antibody
CD98 Background Information
4F2 cell-surface antigen heavy chain, also known as 4F2 heavy chain antigen, Lymphocyte activation antigen 4F2 large subunit, CD98, SLC3A2 and MDU1, is a single-pass type I I membrane protein which belongs to the SLC3A transporter family. SLC3A2 / MDU1 is expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta is significantly stronger at full-term than at the mid-trimester stage. SLC3A2 / MDU1 is expressed in HUVECS and at low levels in resting peripheral blood T-lymphocytes and quiescent fibroblasts. It is expressed in fetal liver and in the astrocytic process of primary astrocytic gliomas. SLC3A2 / MDU1 is also expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco2-BBE. SLC3A2 / MDU1 is required for the function of light chain amino-acid transporters. It involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. SLC3A2 / MDU1 is involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7, SLC3A2 / MDU1 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. SLC3A2 / MDU1 plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. It is required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, SLC3A2 / MDU1 is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta.
Full Name
solute carrier family 3 (amino acid transporter heavy chain), member 2
References
Torrents D. et al., 1998, J Biol Chem. 273: 32437-45. Pfeiffer R. et al., 1999, EMBO J. 18: 49-57. Broeer A. et al., 2000, Biochem J. 349: 787-95. Yanagida O. et al., 2001, Biochim Biophys Acta. 1514: 291-302. Broeer A. et al., 2001, Biochem J. 355: 725-31. Fort J. et al., 2007, J Biol Chem. 282: 31444-52. Mayya V. et al., 2009, Sci Signal. 2: RA46-RA46.
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