|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat PTN ORF mammalian expression plasmid, C-GFPSpark tag||RG81121-ACG|
|Rat PTN ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81121-ACR|
|Rat PTN ORF mammalian expression plasmid, C-Flag tag||RG81121-CF|
|Rat PTN ORF mammalian expression plasmid, C-His tag||RG81121-CH|
|Rat PTN ORF mammalian expression plasmid, C-Myc tag||RG81121-CM|
|Rat PTN ORF mammalian expression plasmid, C-HA tag||RG81121-CY|
|Rat PTN ORF mammalian expression plasmid, N-Flag tag||RG81121-NF|
|Rat PTN ORF mammalian expression plasmid, N-His tag||RG81121-NH|
|Rat PTN ORF mammalian expression plasmid, N-Myc tag||RG81121-NM|
|Rat PTN ORF mammalian expression plasmid, N-HA tag||RG81121-NY|
|Rat PTN Gene cDNA clone plasmid||RG81121-U|
|Rat PTN natural ORF mammalian expression plasmid||RG81121-UT|
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HB-GAM belongs to the pleiotrophin family. During embryonic and early postnatal development, HB-GAM is expressed in the central and peripheral nervous system and also in several non-neural tissues, notably lung, kidney, gut and bone. While in the adult central nervous system, it is expressed in an activity-dependent manner in the hippocampus where it can suppress long term potentiation induction. HB-GAM has a low expression in other areas of the adult brain, but it can be induced by ischemic insults, or targeted neuronal damaged in the entorhinal cortex or in the substantia nigra pars compacta. It is structurally related to midkine and retinoic acid induced heparin-binding protein and has a high affinity for heparin. HB-GAM binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. It also functions as a secreted growth factor and induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells.