|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat FKBP1A ORF mammalian expression plasmid, C-GFPSpark tag||RG81138-ACG|
|Rat FKBP1A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG81138-ACR|
|Rat FKBP1A ORF mammalian expression plasmid, N-GFPSpark tag||RG81138-ANG|
|Rat FKBP1A ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG81138-ANR|
|Rat FKBP1A ORF mammalian expression plasmid, C-Flag tag||RG81138-CF|
|Rat FKBP1A ORF mammalian expression plasmid, C-His tag||RG81138-CH|
|Rat FKBP1A ORF mammalian expression plasmid, C-Myc tag||RG81138-CM|
|Rat FKBP1A ORF mammalian expression plasmid, C-HA tag||RG81138-CY|
|Rat FKBP1A ORF mammalian expression plasmid, N-Flag tag||RG81138-NF|
|Rat FKBP1A ORF mammalian expression plasmid, N-His tag||RG81138-NH|
|Rat FKBP1A ORF mammalian expression plasmid, N-Myc tag||RG81138-NM|
|Rat FKBP1A ORF mammalian expression plasmid, N-HA tag||RG81138-NY|
|Rat FKBP1A Gene cDNA clone plasmid||RG81138-U|
|Rat FKBP1A natural ORF mammalian expression plasmid||RG81138-UT|
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FK506 binding protein 12 (FKBP12), also known as FKBP1, along with cyclophilin, are two major members of the immunophilin protein family who serve as receptors for the immunosuppressant drugs cyclosporin A and FK506. As a conserved molecules in many eukaryotes, FKBP12 has been characterized as a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans-proline residues, and is involved in several biochemical processes including protein folding, receptor signaling, protein trafficking and transcription. FKBP12 has attracted immense attention and its role in mediating the immunosuppressive functions. FKBP12 serves a dual role as a peptidyl-prolyl cis-trans isomerase and as a modulator of several cell signaling pathways. In one such a role, FKBP12 interacts with and regulates the functional state of the ryanodine Ca2+ channel receptor by altering protein conformation and coordinating multi-protein complex formation. Another physiological role of FKBP12 is an interactor and a regulator of the type I serine/threonine kinase receptors of TGF-beta superfamily. Current data, derived from detailed biochemical studies as well as from functional studies in various systems, suggest that FKBP12 functions as a "guardian" for the type I receptors to prevent them from leaky signaling under sub-optimal ligand concentrations, thereby providing a molecular "gradient reader" for TGF-beta family morphogens. This aspect of FKBP12 function may be critical for cellular responsiveness to morphogenetic gradients of the TGF-beta family members during early development, serving to assure the translation of different ligand concentrations into different signaling readouts. In addition, FKBP12 may be involved in neuronal or astrocytic cytoskeletal organization and in the abnormal metabolism of tau protein in Alzheimer's disease (AD) damaged neurons.