|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat FGF9 ORF mammalian expression plasmid, C-GFPSpark tag||RG80143-ACG|
|Rat FGF9 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80143-ACR|
|Rat FGF9 ORF mammalian expression plasmid, C-Flag tag||RG80143-CF|
|Rat FGF9 ORF mammalian expression plasmid, C-His tag||RG80143-CH|
|Rat FGF9 ORF mammalian expression plasmid, C-Myc tag||RG80143-CM|
|Rat FGF9 ORF mammalian expression plasmid, C-HA tag||RG80143-CY|
|Rat FGF9 Gene cDNA clone plasmid||RG80143-G|
|Rat FGF9 ORF mammalian expression plasmid, N-Flag tag||RG80143-NF|
|Rat FGF9 ORF mammalian expression plasmid, N-His tag||RG80143-NH|
|Rat FGF9 ORF mammalian expression plasmid, N-Myc tag||RG80143-NM|
|Rat FGF9 ORF mammalian expression plasmid, N-HA tag||RG80143-NY|
|Rat FGF9 natural ORF mammalian expression plasmid||RG80143-UT|
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Fibroblast growth factor 9 (FGF9) also known as Glia-activating factor or Heparin-binding growth factor 9, is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis. FGF9 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. FGF9 may have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.