|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Maus CCN3 / NOV (IGFBP9) Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||MG50455-ACG|
|Maus CCN3 / NOV (IGFBP9) Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||MG50455-ACR|
|Maus CCN3 / NOV (IGFBP9) Gene ORF cDNA clone expression plasmid, C-Flag tag||MG50455-CF|
|Maus CCN3 / NOV (IGFBP9) Gene ORF cDNA clone expression plasmid, C-His tag||MG50455-CH|
|Maus CCN3 / NOV (IGFBP9) Gene ORF cDNA clone expression plasmid, C-Myc tag||MG50455-CM|
|Maus CCN3 / NOV (IGFBP9) Gene ORF cDNA clone expression plasmid, C-HA tag||MG50455-CY|
|Maus CCN3 / NOV (IGFBP9) Gene ORF cDNA clone in cloning vector||MG50455-M|
|Maus CCN3 / NOV (IGFBP9) Gene ORF cDNA clone expression plasmid, N-Flag tag||MG50455-NF|
|Maus CCN3 / NOV (IGFBP9) Gene ORF cDNA clone expression plasmid, N-His tag||MG50455-NH|
|Maus CCN3 / NOV (IGFBP9) Gene ORF cDNA clone expression plasmid, N-Myc tag||MG50455-NM|
|Maus CCN3 / NOV (IGFBP9) Gene ORF cDNA clone expression plasmid, N-HA tag||MG50455-NY|
|Maus CCN3 / NOV (IGFBP9) Gene ORF cDNA clone expression plasmid||MG50455-UT|
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Protein NOV homolog, also known as Nephroblastoma-overexpressed gene protein homolog, NOV, and CCN3, is a putative ligand for integrin receptors, is tightly associated with the extracellular matrix and mediates diverse cellular functions, including cell adhesion and proliferation. CCN3 has been shown to negatively regulate growth although it promotes migration in a cell type-specific manner. This secreted protein belongs to the CCN family, and its expression was observed in a broad variety of tissues from the early stage of development , and altered expression of CCN3 has been observed in a variety of tumors, including hepatocellular carcinomas, Wilm's tumors, Ewing's sarcomas, gliomas, rhabdomyosarcomas, and adrenocortical carcinomas. Mature CCN3 protein has five distinct modules and truncated protein variants with altered function are found in many cancers. CCN3 acts through the core stem cell signalling pathways including Notch and Bone Morphogenic Protein, connecting CCN3 with the modulation of self-renewal and maturation of a number of cell lineages including hematopoietic, osteogenic and chondrogenic. CCN3 may affect the extracellular environment of the niche for hematopoietic stem cells. CCN3 has emerged as a key player in stem cell regulation, hematopoiesis and a crucial component within the bone marrow microenvironment.