CCR7/CD197 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag

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CCR7/CD197 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag: General Information

Gene
Species
Mouse
NCBI Ref Seq
RefSeq ORF Size
1137 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 327 A/G and 1038 C/T not causing the amino acid variation.
Description
Full length Clone DNA of Mouse chemokine (C-C motif) receptor 7 with C terminal Flag tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
Restriction Sites
KpnI + XbaI (6kb + 1.19kb)
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

CCR7/CD197 cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

CCR7/CD197 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag: Validated Images

The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.

CCR7/CD197 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag: Alternative Names

CC-CKR-7 cDNA ORF Clone, Mouse; CCR-7 cDNA ORF Clone, Mouse; CD197 cDNA ORF Clone, Mouse; Cdw197 cDNA ORF Clone, Mouse; Cmkbr7 cDNA ORF Clone, Mouse; EBI1 cDNA ORF Clone, Mouse; Ebi1h cDNA ORF Clone, Mouse
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