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Mensch MVP/major vault protein Gene ORF cDNA clone in cloning vector

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    Mensch MVP Produktinformation zum cDNA-Klon
    Gene_bank_ref_id:BC015623
    cDNA-Größe:2682bp
    cDNA-Beschreibung:Full length Clone DNA of Homo sapiens major vault protein.
    Synonyme für Gene:LRP, VAULT1
    Spezies:Human
    Vektor:pGEM-T Vector
    Plasmid:pGEM-MVP
    Restriktionsschnittstelle:
    Tag-Sequenz:
    Sequenzbeschreibung:Identical with the Gene Bank Ref. ID sequence except for the point mutations 960 C/T and 1300 C/T not causing the amino acid variation.
    Sequencing primers:SP6 and T7 or M13-47 and RV-M
    ( We provide with MVP qPCR primers for gene expression analysis, HP104572 )
    Promoter:
    Application:
    Antibiotic in E.coli:Ampicillin
    Antibiotic in mammalian cell:
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Lagerung:The lyophilized plasmid can be stored at room temperature for three months.
    pGEM-T Vector Information

    The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

    pGEM-T Simple Usage Suggestion:

    The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

    Vector Sequence Download
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