|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mensch MIF Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||HG10246-ACG|
|Mensch MIF Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||HG10246-ACR|
|Mensch MIF Gene ORF cDNA clone expression plasmid, C-Flag tag||HG10246-CF|
|Mensch MIF Gene ORF cDNA clone expression plasmid, C-His tag||HG10246-CH|
|Mensch MIF Gene ORF cDNA clone expression plasmid, C-Myc tag||HG10246-CM|
|Mensch MIF Gene ORF cDNA clone expression plasmid, C-HA tag||HG10246-CY|
|Mensch MIF Gene ORF cDNA clone in cloning vector||HG10246-M|
|Mensch MIF Gene ORF cDNA clone expression plasmid, N-Flag tag||HG10246-NF|
|Mensch MIF Gene ORF cDNA clone expression plasmid, N-His tag||HG10246-NH|
|Mensch MIF Gene ORF cDNA clone expression plasmid, N-Myc tag||HG10246-NM|
|Mensch MIF Gene ORF cDNA clone expression plasmid, N-HA tag||HG10246-NY|
|Mensch MIF Gene ORF cDNA clone expression plasmid||HG10246-UT|
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Macrophage migration inhibitory factor (MIF) is an immunoregulatory cytokine, the effect of which on arresting random immune cell movement was recognized several decades ago. Despite its historic name, MIF also has a direct chemokine-like function and promotes cell recruitment. MIF is an ubiquitously expressed protein that plays a crucial role in many inflammatory and autoimmune disorders. Increasing evidence suggests that MIF also controls metabolic and inflammatory processes underlying the development of metabolic pathologies associated with obesity. Further research has shown that MIF plays a particularly critical part in cell cycle regulation and therefore in tumorigenesis as well. The significance of the role of MIF in a variety of both solid and hematologic tumors has been established. More recently, interest has increased in the role of MIF in the development of central nervous system (CNS) tumors, in which it appears to influence cell cycle control. MIF contributes to malignant disease progression on several different levels. Both circulating and intracellular MIF protein levels are elevated in cancer patients and MIF expression reportedly correlates with stage, metastatic spread and disease-free survival. Blockade of MIF bioactivity successfully inhibited tumor cell growth in vivo and in vitro. MIF plays important roles in the pathogenesis of gastrointestinal, hepatic, and pancreatic disorders.