|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human VWC2 ORF mammalian expression plasmid, C-GFPSpark tag||HG12071-ACG|
|Human VWC2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12071-ACR|
|Human VWC2 ORF mammalian expression plasmid, C-Flag tag||HG12071-CF|
|Human VWC2 ORF mammalian expression plasmid, C-His tag||HG12071-CH|
|Human VWC2 ORF mammalian expression plasmid, C-Myc tag||HG12071-CM|
|Human VWC2 ORF mammalian expression plasmid, C-HA tag||HG12071-CY|
|Human VWC2 Gene cDNA clone plasmid||HG12071-G|
|Human VWC2 ORF mammalian expression plasmid, N-Flag tag||HG12071-NF|
|Human VWC2 ORF mammalian expression plasmid, N-His tag||HG12071-NH|
|Human VWC2 ORF mammalian expression plasmid, N-Myc tag||HG12071-NM|
|Human VWC2 ORF mammalian expression plasmid, N-HA tag||HG12071-NY|
|Human VWC2 natural ORF mammalian expression plasmid||HG12071-UT|
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Brorin, also known as brain-specific chordin-like protein, von Willebrand factor C domain-containing protein 2 and VWC2, is a secreted protein which contains two VWFC domains. VWC2 / Brorin is a BMP antagonist which may play a role in neural development. It promotes cell adhesion. VWC2 / Brorin is a unique member of the chordin family. It inhibited the activity of bone morphogenetic protein 2 (BMP2) and BMP6 in mouse preosteoblastic MC3T3-E1 cells. Mouse Brorin was predominantly expressed in neural tissues in embryos and also predominantly expressed in the adult brain. In the brain, the expression was detected in neurons, but not glial cells. The neural tissue-specific expression profile of Brorin is quite distinct from that of any other member of the Chordin family. VWC2 / Brorin protein promoted neurogenesis, but not astrogenesis, in mouse neural precursor cells. VWC2 / Brorin is a novel secreted BMP antagonist that potentially plays roles in neural development and functions.