Human VSIG8 HEK293 Overexpression Lysate: Product Information
This Human VSIG8 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of VSIG8 protein (Cat: 15654-H02H) from the overexpression lysate was verified.
A DNA sequence encoding the extracellular domain of human VSIG8 (NP_001013683.1)(Met1-Gly263) was expressed with the Fc region of human IgG1 at the C-terminus.
The recombinant human VSIG8/Fc comprises 480 amino acids and has a predicted molecular mass of 53.8 kDa. The apparent molecular mass of the protein is approximately 55.9 kDa in SDS-PAGE under reducing conditions.
Human VSIG8 HEK293 Overexpression Lysate: Usage Guide
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human VSIG8 HEK293 Overexpression Lysate: Alternative Names
Human C1orf204 Overexpression Lysate
VSIG8 Background Information
V-set and immunoglobulin domain containing 8 (VSIG8) is essential in hair cycle, oral mucosa, and the nail matrix. Moreover, VSIG8 also has an important role in proper epithelial differentiation and function in the upper alimentary tract.
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