|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human TNFα ORF mammalian expression plasmid, C-GFPSpark tag||HG10602-ACG|
|Human TNFα ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10602-ACR|
|Human TNFα ORF mammalian expression plasmid, C-Flag tag||HG10602-CF|
|Human TNFα ORF mammalian expression plasmid, C-His tag||HG10602-CH|
|Human TNFα ORF mammalian expression plasmid, C-Myc tag||HG10602-CM|
|Human TNFα ORF mammalian expression plasmid, C-HA tag||HG10602-CY|
|Human TNFα ORF mammalian expression plasmid, N-Flag tag||HG10602-NF|
|Human TNFα ORF mammalian expression plasmid, N-His tag||HG10602-NH|
|Human TNFα ORF mammalian expression plasmid, N-Myc tag||HG10602-NM|
|Human TNFα ORF mammalian expression plasmid, N-HA tag||HG10602-NY|
|Human TNFα natural ORF mammalian expression plasmid||HG10602-UT|
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Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.