|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human LAMP2 ORF mammalian expression plasmid, C-GFPSpark tag||HG13555-ACG|
|Human LAMP2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13555-ACR|
|Human LAMP2 ORF mammalian expression plasmid, C-Flag tag||HG13555-CF|
|Human LAMP2 ORF mammalian expression plasmid, C-His tag||HG13555-CH|
|Human LAMP2 ORF mammalian expression plasmid, C-Myc tag||HG13555-CM|
|Human LAMP2 ORF mammalian expression plasmid, C-HA tag||HG13555-CY|
|Human LAMP2 Gene cDNA clone plasmid||HG13555-G|
|Human LAMP2 ORF mammalian expression plasmid, N-Flag tag||HG13555-NF|
|Human LAMP2 ORF mammalian expression plasmid, N-His tag||HG13555-NH|
|Human LAMP2 ORF mammalian expression plasmid, N-Myc tag||HG13555-NM|
|Human LAMP2 ORF mammalian expression plasmid, N-HA tag||HG13555-NY|
|Human LAMP2 natural ORF mammalian expression plasmid||HG13555-UT|
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LAMP2 (Lysosomal-associated membrane protein 2), also known as CD107b (Cluster of Differentiation 107b), is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. In human, LAMP2, the causative gene of Danon disease, located on chromosome Xq24, encodes the lysosome-associated membrane protein-2 (LAMP-2). LAMP-2 deficiency, or Danon disease, is a rare X-linked lysosomal disease characterized by cardiomyopathy, vacuolar myopathy, and mental retardation. LAMP2 cardiomyopathy is an X-linked and highly progressive myocardial storage disorder associated with diminished survival, which clinically resembles sarcomeric hypertrophic cardiomyopathy.