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Mensch CIRBP / Cold-inducible RNA-binding Protein Gene ORF cDNA clone expression plasmid, N-Flag tag

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Human CIRBP Produktinformation zum cDNA-Klon
Gene_bank_ref_id:BC000403
cDNA-Größe:519bp
cDNA-Beschreibung:Full length Clone DNA of Homo sapiens cold inducible RNA binding protein with N terminal Flag tag.
Synonyme für Gene:CIRP
Spezies:Human
Vektor:pCMV3-N-FLAG
Plasmid:
Restriktionsschnittstelle:
Tag-Sequenz:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequenzbeschreibung:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Lagerung:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Mensch CIRBP / Cold-inducible RNA-binding Protein Gene ORF cDNA clone expression plasmid, N-Flag tag on other vectors
Mensch CIRBP / Cold-inducible RNA-binding Protein Gene ORF cDNA clone expression plasmid, C-GFPSpark tagHG14578-ACG$225
Mensch CIRBP / Cold-inducible RNA-binding Protein Gene ORF cDNA clone expression plasmid, C-OFPSpark tagHG14578-ACR$225
Mensch CIRBP / Cold-inducible RNA-binding Protein Gene ORF cDNA clone expression plasmid, N-GFPSpark tagHG14578-ANG$225
Mensch CIRBP / Cold-inducible RNA-binding Protein Gene ORF cDNA clone expression plasmid, N-OFPSpark tagHG14578-ANR$225
Mensch CIRBP / Cold-inducible RNA-binding Protein Gene ORF cDNA clone expression plasmid, C-Flag tagHG14578-CF$195
Mensch CIRBP / Cold-inducible RNA-binding Protein Gene ORF cDNA clone expression plasmid, C-His tagHG14578-CH$195
Mensch CIRBP / Cold-inducible RNA-binding Protein Gene ORF cDNA clone expression plasmid, C-Myc tagHG14578-CM$195
Mensch CIRBP / Cold-inducible RNA-binding Protein Gene ORF cDNA clone expression plasmid, C-HA tagHG14578-CY$195
Mensch CIRBP / Cold-inducible RNA-binding Protein Gene ORF cDNA clone in cloning vectorHG14578-G$75
Mensch CIRBP / Cold-inducible RNA-binding Protein Gene ORF cDNA clone expression plasmid, N-Flag tagHG14578-NF$195
Mensch CIRBP / Cold-inducible RNA-binding Protein Gene ORF cDNA clone expression plasmid, N-His tagHG14578-NH$195
Mensch CIRBP / Cold-inducible RNA-binding Protein Gene ORF cDNA clone expression plasmid, N-Myc tagHG14578-NM$195
Mensch CIRBP / Cold-inducible RNA-binding Protein Gene ORF cDNA clone expression plasmid, N-HA tagHG14578-NY$195
Mensch CIRBP / Cold-inducible RNA-binding Protein Gene ORF cDNA clone expression plasmidHG14578-UT$195
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Hintergrund

CIRBP, also known as cold-inducible RNA-binding protein, plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. CIRBP responds to a wide array of cellular stresses, including short wavelength ultraviolet light (UVC), at the transcriptional and post-translational level. It acts as a translational activator.CIRBP can bind the 3 translated region of specific transcripts to stabilize them and facilitate their transport to ribosomes for translation. CIRBP affects NF-κB signaling as opposed to IL1B mRNA stability directly.

Referenzen
  • Artero-Castro A. et al., 2009, Mol Cell Biol. 29 (7): 1855-68.
  • Zeng Y. et al., 2009, J Cell Biochem. 107 (1): 179-88.
  • Brochu C. et al., 2013, PLoS One. 8 (2): e57426.
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    Katalog: HG14578-NF
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    Verfügbarkeit2-3 weeks
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