|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human CA III ORF mammalian expression plasmid, C-GFPSpark tag||HG10503-ACG|
|Human CA III ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10503-ACR|
|Human CA III ORF mammalian expression plasmid, N-GFPSpark tag||HG10503-ANG|
|Human CA III ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10503-ANR|
|Human CA III ORF mammalian expression plasmid, C-Flag tag||HG10503-CF|
|Human CA III ORF mammalian expression plasmid, C-His tag||HG10503-CH|
|Human CA III ORF mammalian expression plasmid, C-Myc tag||HG10503-CM|
|Human CA III ORF mammalian expression plasmid, C-HA tag||HG10503-CY|
|人 CA III 基因全长ORF克隆||HG10503-M|
|Human CA III ORF mammalian expression plasmid, N-Flag tag||HG10503-NF|
|Human CA III ORF mammalian expression plasmid, N-His tag||HG10503-NH|
|Human CA III ORF mammalian expression plasmid, N-Myc tag||HG10503-NM|
|Human CA III ORF mammalian expression plasmid, N-HA tag||HG10503-NY|
|Human CA III natural ORF mammalian expression plasmid||HG10503-UT|
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Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases (CAs) form a family of enzymes that catalyze the rapid conversion of carbon dioxide and water to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes. Several forms of carbonic anhydrase occur in nature. The primary function of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. Plants contain a different form called β-carbonic anhydrase, which, from an evolutionary standpoint, is a distinct enzyme, but participates in the same reaction and also uses a zinc ion in its active site.
Carbonic anhydrase 3, also known as Carbonate dehydratase III, CA-III and CA3, is a cytoplasm protein which belongs to the alpha-carbonic anhydrase family. CA3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine. It is inhibited by coumarins and sulfonamide derivatives such as acetazolamide. At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.