Frizzled 10 Protein, Human, Recombinant (His Tag)

Cat: 16045-H08H

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Frizzled 10 Protein, Human, Recombinant (His Tag) Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the human FZD10 (NP_009128.1) (Met1-Gly161) was expressed with a polyhistidine tag at the C-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Ile 21
Molecule Mass
The recombinant human FZD10 consists of 152 amino acids and predicts a molecular mass of 17.5 KDa. It migrates as an approximately 25.4 KDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Frizzled 10 Protein, Human, Recombinant (His Tag) in SDS-PAGE

Human Frizzled-10/FZD10 (CD350) Protein 10919

Frizzled 10 Protein, Human, Recombinant (His Tag) Alternative Names

CD350 Protein, Human;FZ-10 Protein, Human;Fz10 Protein, Human;FzE7 Protein, Human;hFz10 Protein, Human

Frizzled 10 Background Information

Frizzled-10, also known as Fz-10, CD350 and FZD10, is a multi-pass membrane protein which belongs to the G-protein coupled receptor Fz/Smo family. Frizzled-10 / FZD10 is abundantly expressed in the cerebellum, followed by cerebral cortex, medulla and spinal cord; very low levels in total brain, frontal lobe, temporal lobe and putamen. It is weakly expressed in adult brain, heart, lung, skeletal muscle, pancreas, spleen and prostate. Frizzled-10 / FZD10 is a receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Frizzled-10 / FZD10 may also be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

Full Name
frizzled class receptor 10
References
  • Kwon H.S., et al.,(2009), Myocilin is a modulator of Wnt signaling. Mol. Cell. Biol. 29:2139-2154.
  • The MGC Project Team, et al., (2004), The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).Genome Res. 14:2121-2127.
  • Tanaka S., et al.,(1998), A novel frizzled gene identified in human esophageal carcinoma mediates APC/beta-catenin signals.Proc. Natl. Acad. Sci. U.S.A. 95:10164-10169.
  • Other Frizzled 10 Proteins

    Mouse Frizzled 10 Proteins

    Product Name Cat.No.
    Frizzled 10 Protein, Mouse, Recombinant (Fc Tag) 50568-M02H
    Frizzled 10 Protein, Mouse, Recombinant (His Tag) 50568-M08H

    Human Frizzled 10 Proteins

    Product Name Cat.No.
    Frizzled 10 Protein, Human, Recombinant (Fc Tag) 16045-H02H
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