Anti-ssPA Antibody

Cat: 13098-RP02
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Anti-ssPA Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-ssPA Antibody
Validated applications
ELISA
Species reactivity
Reacts with: E. coli
Specificity
E. coli ssPA
Immunogen
Recombinant Escherichia coli ssPA / Stringent starvation protein A protein (Catalog#13098-E07E)
Preparation
Produced in rabbits immunized with purified, recombinant Escherichia coli Escherichia coli ssPA / Stringent starvation protein A (Catalog#13098-E07E; P0ACA3; Met 1-Ser 212). Escherichia coli ssPA / Stringent starvation protein A specific IgG was purified by Escherichia coli Escherichia coli ssPA / Stringent starvation protein A affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-ssPA Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:5000-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-ssPA Antibody Alternative Names
Anti-pog Antibody;Anti-ssp Antibody
ssPA Background Information

Stringent starvation protein A (ssPA), a member of the GST superfamily, is an RNA polymerase-associated transcriptional activator for the lytic development of phage P1 and is essential for stationary phase-induced acid tolerance of E. coli. It indicate that the flexible regions are not critical for SspA function, whereas the surface pocket is important for both transcriptional activation of the phage P1 late promoter and acid resistance of E. coli. SspA is known to be implicated in survival during nutrient starvation and prolonged stationary phase. Recently, SspA was shown to play an important role in the stationary phase-induced stress response including acid tolerance by down-regulating the level of the global regulator H-NS, which negatively regulates multiple stress defense systems.

References
  • Hansen AM, et al. (2003) Escherichia coli SspA is a transcription activator for bacteriophage P1 late genes. Mol Microbiol. 48(6): 1621-31.
  • Hommais F, et al. (2001) Large-scale monitoring of pleiotropic regulation of gene expression by the prokaryotic nucleoid-associated protein, H-NS. Mol Microbiol. 40(1): 20-36.
  • Atlung T, et al. (1997) H-NS: a modulator of environmentally regulated gene expression. Mol Microbiol. 24(1): 7-17.
  • Williams MD, et al. (1994) Starvation-induced expression of SspA and SspB: the effects of a null mutation in sspA on Escherichia coli protein synthesis and survival during growth and prolonged starvation. Mol Microbiol. 11(6): 1029-43.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-ssPA Antibody Rabbit Polyclonal ELISA 13098-RP01
    Anti-ssPA Antibody Rabbit Monoclonal ELISA 13098-R113
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