Anti-E-Selectin Antibody (PE)

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Anti-E-Selectin Antibody (PE) (Mouse Monoclonal antibody) General Information

Product name
Anti-E-Selectin Antibody (PE)
Validated applications
FCM
Species reactivity
Reacts with: Human
Specificity
Human E-Selectin
Immunogen
Recombinant Human E-Selectin / CD62e protein (Catalog#10335-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human E-Selectin / CD62e (rh E-Selectin / CD62e; Catalog#10335-H08H; NP_000441.2; Met 1-Pro 556) and conjugated with PE under optimum conditions, the unreacted PE was removed.
Source
Monoclonal Mouse IgG2b Clone #10
Purification
Protein A
Formulation
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Conjugate
PE
Concentration
10 μl/Test, 0.1 mg/ml
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Anti-E-Selectin Antibody (PE) (Mouse Monoclonal antibody) Images

Flow cytometric analysis of Human CD62E in HUVEC cells. HUVEC cells (Human umbilical vein endothelial cells) were treated with TNF-α (50 ng/ml, 18 hours). The cells were harvested and stained with PE Mouse anti-Human CD62E (10335-MM10-P).

Anti-E-Selectin Antibody (PE): Alternative Names

Anti-CD62E Antibody; Anti-ELAM Antibody; Anti-ELAM1 Antibody; Anti-ESEL Antibody; Anti-LECAM2 Antibody

E-Selectin Background Information

E-selectin, also known as endothelial leukocyte adhesion molecule-1 (ELAM-1) and CD62E, is an inducible adhesion molecule that is expressed on the surfaces of stimulated vascular endothelial cells and is sometimes involved in cancer cell metastasis. E-selectin exhibits a complex mosaic structure consisting of a large extracellular region comprised of a lectin domain, an EGF-like domain, and a short consensus repeat (SCR) domain, followed by a transmembrane region and a relatively short (32 aa) cytoplasmic tail. As a member of the LEC-CAM or selectin family, E-selectin recognises and binds to sialylated carbohydrates including members of the Lewis X and Lewis A families found on monocytes, granulocytes, and T-lymphocytes. E-selectin supports rolling and stable arrest of leukocytes on activated vascular endothelium, and furthermore, it was indicated that it can also transduce an activating stimulus via the MAPK cascade into the endothelial cell during leukocyte adhesion. E-selectin regulates adhesive interactions between certain blood cells and endothelium. The soluble form of E selectin (sE-selectin) is a marker of endothelial activation, and has a potential role in the pathogenesis of cardiovascular disease as raised levels have been found in hypertension, diabetes and hyperlipidemia, although its association in established atherosclerosis disease and its value as a prognostic factor is more controversial. soluble E-selectin is inversely associated with the muscular component of the left ventricle, thereby suggesting that the lack of such a reparative factor may be associated with cardiac remodeling in end-stage renal disease (ESRD) patients. In addition, this adhesion molecule appears to be involved in the pathogenesis of atherosclerosis.
Full Name
selectin E
References
  • Roldn V, et al. (2003) Soluble E-selectin in cardiovascular disease and its risk factors. A review of the literature. Thromb Haemost. 90(6): 1007-20.
  • Kawase J, et al. (2009) Increase in E-selectin expression in umbilical vein endothelial cells by anticancer drugs and inhibition by cimetidine. Oncol Rep. 22(6): 1293-7.
  • Matsumoto K, et al. (2010) Soluble adhesion molecule E-selectin predicts cardiovascular events in Japanese patients with type 2 diabetes mellitus. Metabolism. 59(3): 320-4.
  • Stancanelli B, et al. (2010) Soluble e-selectin is an inverse and independent predictor of left ventricular wall thickness in end-stage renal disease patients. Nephron Clin Pract. 114(1): c74-80.

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