Anti-CA5A Antibody

Cat: 10500-RP02

Contact us:

215-583-7898
order_us@sinobiological.com
Distributors

All CA5A Reagents

CRO Services

Product recommended


All CA5A Reagents

Anti-CA5A Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-CA5A Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Human
Specificity
Human CA5A
Immunogen
Recombinant Human Carbonic Anhydrase VA / CA5A protein (Catalog#10500-H08E)
Preparation
Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase VA / CA5A (rh Carbonic Anhydrase VA / CA5A; Catalog#10500-H08E; NP_001730.1; Ala 40-Ser 305). Carbonic Anhydrase VA / CA5A specific IgG was purified by Human Carbonic Anhydrase VA / CA5A affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-CA5A Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CA5A Antibody (Rabbit Polyclonal antibody) Images

Human Carbonic Anhydrase VA/CA5A Western blot (WB) 13240

Anti-CA5A rabbit polyclonal antibody at 1:500 dilution

Lane A: NCI-H460 Whole Cell Lysate

Lane B: A549 Whole Cell Lysate

Lysates/proteins at 60 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:35 kDa

Observed band size:35 kDa

(We are unsure as to the identity of these extra bands.)

Anti-CA5A Antibody Alternative Names

Anti-CA5 Antibody;Anti-CA5AD Antibody;Anti-CA5D Antibody;Anti-Carbonic Anhydrase VA Antibody;Anti-CAV Antibody;Anti-CAVA Antibody;Anti-GS1-21A4.1 Antibody

CA5A Background Information

Carbonic anhydrase 5A, mitochondrial, also known as Carbonate dehydratase VA, Carbonic anhydrase VA, CA-VA and CA5A, is a member of the alpha-carbonic anhydrase family. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA5A / CA-VA is activated by histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. It is inhibited by coumarins, sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt).

Full Name
carbonic anhydrase VA, mitochondrial
References
  • Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99:16899 - 903.
  • Liao, S.Y. et al., 2003, J. Med. Genet. 40:257 - 262.
  • Temperini C.et al., 2006, Chemistry 12: 7057-66.
  • Temperini C.et al., 2006, J. Med. Chem. 49: 3019-27.
  • Supuran, C. T. et al., 2008, Curr Pharm Des. 14 (7): 601-602.
  • Elleuche, S. et al., 2009, Curr Genet. 55 (2): 211-222.
  • Product Description Host Clonality Application Catalog# (PDF)
    info info
    添加购物车成功! 添加购物车失败!